VqMAPK3/VqMAPK6, VqWRKY33, and <i>VqNSTS3</i> consist of a regulatory node in enhancing resistance to powdery mildew in grapevine
نویسندگان
چکیده
Abstract Grapevine powdery mildew is caused by Erysiphe necator that seriously harms grape production in the world. Stilbene synthase makes phytoalexins contribute to resistance of grapevine against mildew. A novel VqNSTS3 was identified and cloned from Chinese wild Vitis quinquangularis accession Danfeng-2. Novel transferred into susceptible ‘Thompson Seedless’ Agrobacterium-mediated transformation. The transgenic plants showed disease activated other resistance-related genes. expression regulated VqWRKY33, which binds TTGACC promoter with fungal elicitor responsive element. Furthermore, VqWRKY33 phosphorylated VqMAPK3/VqMAPK6 thus lead enhanced signal transduction increased expression. ProVqNSTS3::VqNSTS3-GFP Arabidopsis thaliana observed move wrap pathogen haustoria block invasion Golovinomyces cichoracearum. These results demonstrated stilbene accumulation a prevented Therefore, can be used generating mildew-resistant grapevines.
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................................................ 2 Executive summary .................................................. 3 Background ................................................. 5 Project Aims & Performance targets .................................................. 6 Methods ................................................. 7 Results / Discussion ..............................................
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ژورنال
عنوان ژورنال: Horticulture research
سال: 2023
ISSN: ['2052-7276', '2662-6810']
DOI: https://doi.org/10.1093/hr/uhad116